Step Number	Task	Methodology	Concepts Taught
1	Acetylate microperoxidase-8	ultrafiltration, dialysis, pH meter	weighing, stoichiometry, acid-base, buffers, pH, ultrafiltration, dialysis
2	Prove peptide is acetylated (color test)	Ninhydrin reaction	amino acids, peptides
3	Purify product by HPLC	Perfusion ion exchange HPLC	chromatography, ion exchange, HPLC revisit buffers and pH, chromophore, UV-vis, absorbance
4	Demonstrate product purity	Electrospray mass spectrometry	ionization, mass spectrometry, electrospray
5	Characterize electronic structure of product	UV-vis	coordination number, spin state, charge, revisit chromophore, absorbance, and UV-vis
6	Characterize product electrochemically in aqueous solution	Cyclic voltammetry	statistics, mass transport, redox, reversibility
7	Characterize product electrochemically in dimethyl sulfoxide	Cyclic voltammetry	revisit statistics, mass transport, redox, reversibility